Binding Strength

Kd and occupancy

An interactive lesson with 5 steps, about 5 minutes: you predict, drag and solve, with instant feedback on every answer. It runs in your browser and needs JavaScript.

What you’ll learn

• Kd: concentration at half occupancy; smaller is tighter.
• θ = [L]/([L] + Kd); 90% needs 9 × Kd.
• Each 10× in Kd ≈ 5.9 kJ/mol in ΔG at 37 °C.

Steps

1. How tight?
2. Never still
3. Ninety percent occupied
4. Ten times tighter
5. From energy to Kd